Lysis Buffer (500mM NaCl)

Mix all reagents, store at 4C. Add PIC and PMSF immediately before use.50ml302.5ml 1M HEPES pH7.55ml 5M NaCl100ul 500mM EDTA5ml 10% Triton X-100250ul 20% SDS0.5ml 10% Socium deoxycholate0.5ml 100X Complete Protease Inhibitor Complex (PIC)0.5ml 100mM PMSF35.65ml H20