Making Antibodies

Raising anti-peptide antibodies in rabbit:

- synthesize peptide of choice, including a Cys residue at the N- or C-terminus

- conjugate peptide to Imject Maleimide Activated Ovalbumin (Pierce) and purify by gel filtration [follow protocol provided with reagent].

- inject young male rabbits with 250ug peptide-ovalbumin conjugate emulsified with 0.5ml Freund’s Complete.

- Boost by repeating the injections at 21 days.

- Obtain antiserum at 28 days.