Peptide Labeling with Maleimide

1) Resuspend peptide in H20 + TCEP to ~400ul

2) HPLC purify, selecting appropriate peak after MS

3) Lyophylize peptide

4) Resuspend in 100ul PBS

5) Add 1-10x molar ratio of maleimide-linked fluorophore

6) Allow reaction to proceed o/n at 4C (in dark)

7) HPLC purify fluorescent peptides

8) Lyophylized peptides can be stored at 4C.