Southern Membrane Stripping

Stripping Southern blots:

- do not allow membranes to dry out between hybridization and stripping
- make up 2L of strip solution (0.1X SSC, 0.1% SDS): 10ml 20X SSC 10ml 20% SDS 1,980ml water
- heat wash solution to 90-100°C
- add 1/3 to filters, incubate for15 minutes on rotating platform, with perspex cover over tub
- repeat x 2
- prehyb as normal