Turkey Erythrocyte Nuclei Isolation

All buffers are chilled on ice and contain protease inhibitors
7.5 mL turkey blood
Wash 3X with PBS
Wash 3X with RSB 50-6 + 1% Triton X-100 or 0.4% NP-40
Wash 3X with RSB 50-6 (no detergent)
Wash 2X with RSB 10-3 + 1mM CaCl2
Resuspend in 1 mL RSB 10-3 + 1mM CaCl2 (A260 approx. 200), use 1 mL of this solution
RSB 50-6 = 50 mM NaCl, 10 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 0.5 mM EGTA
RSB 10-3 + 1mM CaCl2 = 10 mM NaCl, 10 mM Tris-HCl (pH 7.5), 3 mM MgCl2, 1 mM CaCl2