Paper published by Pascal Egea in Protein Science

Paper published by Pascal Egea in Protein Science

The Egea lab publishes a paper titled "Structural mapping of the ClpB ATPases of Plasmodium falciparum: targeting protein folding and secretion for antimalarial drug design" in Protein Science.

By Andrew P. AhYoung, Antoine Koehl, Duilio Cascio and Pascal F. Egea

The eukaryotic parasite Plasmodium falciparum, the causative agent of malaria, has evolved an elaborate network of Clp proteins including two distinct ClpB ATPases. ClpB1 and ClpB2 are involved in different aspects of parasitic proteostasis. ClpB1 is present in the apicoplast, a parasite-specific and plastid-like organelle hosting various metabolic pathways necessary for parasite growth. ClpB2 localizes to the parasitophorous vacuole membrane where it drives protein export as core subunit of a parasite-derived protein secretion complex, the Plasmodium Translocon of Exported proteins (PTEX); this process is central to parasite virulence and survival in the human host. The functional associations of these two chaperones with parasite-specific metabolism and protein secretion make them prime drug targets. In this article, AhYoung et al. report and compare the first crystal structures of the N terminal domains of ClpB1 and ClpB2 from Plasmodium and analyze their molecular surfaces. These structures represent the first step towards the characterization of these two malarial chaperones and the reconstitution of the entire PTEX to aid structure-based design of novel anti-malarial drugs.

The complete article can be found here.