Project Scientist

Project Scientist

NOW HIRING! Project Scientist

Project Scientist
The Department of Biological Chemistry (http://www.biolchem.ucla.edu/), a basic science department within the UCLA School of Medicine, invites highly qualified applications for positions as an Project Scientist. 
Primary duties include leading a project on the development of a molecular diagnostic for Parkinson’s disease, using MRI.  Tasks include, but are not limited to:
1)  supervising and training students in support of the project;
2)  working with collaborators in project development and implementation of goals;
3)  rationally designing fibril-capping peptides for alpha-synuclein fibrils;
3)  developing and implementing large-scale screening methods for identifying novel binders of alpha-synuclein fibrils;
4)  Performing surface plasmon resonance (SPR) experiments to determine binding constants of novel binding peptides;
5)  Testing peptides for their ability to inhibit seeding of alpha-synuclein in HEK293T biosensor cells;
6)  Conjugating peptides to magnetic nanoparticles to form functionalized magnetic nanoparticles (fMNPs);
7)  Testing fMNPs in biosensor cells for their ability to colocalize with alpha-synuclein aggregates and produce a magnetic resonance signal;
8)  Performing electron microscopy to confirm inhibition of fibril formation;
9)  Purifying protein, as needed;
10) Analyzing large data sets generated from next-generation sequencing (NGS);
11)  Performing magnetic and flow cytometry cell sorting in the identification of novel alpha-synuclein fibril binders;
12)  Implementing new techniques and collaborations, as needed, to further the goals of the project.
13)  Writing project reports and presenting research findings at meetings
 
80% A. TECHNICAL RESPONSIBILITIES
 
1. Lead research project with alpha-synuclein fibrils and fibril-capping peptides in the development of a Parkinson’s disease diagnostic.
2. Identify novel fibril-capping peptideserform surface plasmon resonance (SPR) binding studies on amyloid fibrils with fibril-capping peptides.
3. Test functionalized magnetic nano-particles (fMNP) for binding of amyloid fibrils using SPR.
Perform binding studies of fMNPs in specialized HEK293T cells.
4. Optimize fibril-capping peptides, as necessary, to optimize binding and specificity.
5. Perform toxicity testing of peptides and fMNPs on cells.
6. Develop new techniques as needed.
7. Record daily experiments and keep detailed notebooks.
8. Present experimental results at weekly group meetings
9. Prepare publications 
15% B. ADMINISTRATIVE FUNCTIONS
1. Attend weekly laboratory meetings.
2. Abide by any procedural changes, policy changes, laboratory safety and general information.
3. Order needed chemicals and supplies.
4. Common laboratory duties for common equipment as assigned by PI
5% C. CONTINUING EDUCATION
1. Attend relevant scientific meetings and present research results
 
Contact: Cindy Chau 
cchau@mbi.ucla.edu
(310) 825-3754