Research Internship in Dr. Egea’s Lab.
Biochemical and structural studies of eukaryotic protein complexes involved in the transport of lipids and proteins across membranes. Subcloning of genes for protein expression in bacterial and yeast expression vectors. Site-directed mutagenesis. Protein expression in Escherichia coli and Saccharomyces cerevisiae. Protein purification by affinity chromatography, ion-exchange and size-exclusion chromatograhy. Protein characterization. Protein crystallization for 3D structure determination by X-ray crystallography. The protein complexes I am studying are linked to diseases.
Studying at Chime Paristech in Paris.