Biological Chemistry Imaging Facility (BCIF) provides resources for data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. BCIF started operations in December 1994 as a small departmental facility. Over the years it grew into a core imaging facility serving the majority of departments and subdivisions of the UCLA David Geffen School of Medicine.
BCIF provides round-the-clock access to a cluster of fluorescent scanning equipment such as Typhoons 9410 and 9400 Variable Mode Imagers, Licor Odyssey near infra red imager, as well as Gel Documentation systems, color printers, and high-resolution flat-bed scanners. In addition BCIF provides scientific data storage space on its secure storage arrays to all participating labs, as well as access to a number of software packages for data analysis and digital data processing.
All equipment in the facility is available 24 hours a day, 7 days a week. The facility operates on a walk-in basis and provides technical support and instructions during weekdays, primarily for new users. Each lab is provided with its own account and space on a file server. Any lab wishing to establish a new account is welcome to do so by filling out the BCIF Access Request Form and contacting the ITC helpdesk (ITCsupport@mednet.ucla.edu or ext. 49482) to set-up an appointment. For any questions, please contact Elizabeth Duran (firstname.lastname@example.org or x56545)
BCIF has two locations: 344C BSRB for labs located at the Bioscience Research Building and 33-089 CHS for labs located at other campus buildings.
The cost is as follows:
Digital Imaging Processing (unlimited use of lab) - $25/mo per lab
Data File Storage-$5 per 500GB/mo per lab
B&W Printing/Scanning - $0.07/print
Color Printing/Scanning - $0.20/print
Typhoon™ 9410 and 9400 Variable Mode Imagers from GE Healthcare are the main equipment pieces at both BCIF locations. Typhoon imagers are versatile systems, which handle agarose and polyacrylamide gels, membranes, mounted or unmounted storage phosphor screens up to 35×43cm, microplates, microarrays, and in situ slides.
Important Typhoon features:
35 × 43 cm
Sensitivity (limit of detection)
Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): <2 dpm/mm2;32P: 5-10-fold lower than 14C
1000, 500, 200, 100, 50, 25 (9400 model), and 10 µm (9410 model), selectable
Autoradiography:2 line pairs/mm for lines drawn with14C ink.
± 5% over entire scan area.
16-bit (65,536 levels) TIFF
Linear dynamic range and linearity
Five orders of magnitude (100,000:1) with less than 7.5% relative standard deviation for entire dynamic range.
Red light source
Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10,000 h Wavelength: 632.8 nm
Green light source
Type: 20 mW solid-state doubled frequency SYAG laser Estimated average lifetime: ~10.000 h Wavelength: 532 nm
Blue light source
Type: 30 mW all blue lines Argon ion laser Estimated average lifetime: ~5,000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW)
10 Base-T Ethernet using TCP/IP protocol
Scan control software for Windows™ operating system.
115/230 V (switchable), 50-60 Hz, < 500 W
Instrument: 118 × 78 × 48 cm