Biological Chemistry Imaging Facility (BCIF) provides resources for data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. BCIF started operations in December 1994 as a small departmental facility. Over the years it grew into a core imaging facility serving the majority of departments and subdivisions of the UCLA David Geffen School of Medicine.
BCIF provides round-the-clock access to a cluster of fluorescent scanning equipment such as Typhoons 9410 and 9400 Variable Mode Imagers, Licor Odyssey near infra red imager, as well as Gel Documentation systems, color printers, and high-resolution flat-bed scanners. In addition BCIF provides scientific data storage space on its
secure storage arrays to all participating labs, as well as access to a number of software packages for data analysis and digital data processing.
All equipment in the facility is available 24 hours a day, 7 days a week. The facility operates on a walk-in basis and provides technical support and instructions during weekdays, primarily for new users. Each lab is provided with its own account and space on a file server.
The Biological Chemistry Imaging Facility (BCIF) is under the David Geffen School of Medicine (DGSOM) umbrella and converted to AD / MEDNET only facility as of Monday, February 27, 2017.
A MEDNET email address is mandatory to get computer access. If you do not have a MEDNET email address, please complete one of the MEDNET email access forms and submit it to the appropriate office as referenced below.
School of Medicine Departments
NOTE: A MEDNET email is typically generated within 3 business days
Please bring the following to 310 BSRB for access:
- UCLA ID
- BCIF Access Request Form (one form per user)
- For server access, contact DGIT helpdesk (ITCsupport@mednet.ucla.edu) to set-up an appointment. Access is typically granted within 2 business days.
- Questions? Please contact Nicole Vaona (NVaona@mednet.ucla.edu or x56545)
BCIF has one location: 344C BSRB for labs located at the Bioscience Research Building
The cost is as follows:
B&W Printing/Scanning – $0.07/print
Color Printing/Scanning – $0.20/print
Typhoon™ 9410 and 9400 Variable Mode Imagers from GE Healthcare are the main equipment pieces at both BCIF locations. Typhoon imagers are versatile systems, which handle agarose and polyacrylamide gels, membranes, mounted or unmounted storage phosphor screens up to 35×43cm, microplates, microarrays, and in situ slides.
- Storage phosphor
- Direct blue-excited fluorescence (457 nm, 488 nm)
- Direct green-excited fluorescence (532 nm)
- Direct red-excited fluorescence (633 nm)
Important Typhoon features:
- Unique Typhoon property: Highly sensitive confocal optics enables direct and highly quantitative imaging of fluorescent Western blots without intermediate exposure to films or screens
- Powerful excitation sources and confocal optics allow for the sensitive detection of very low-abundance targets
- Red-, green-, and two blue-excitation wavelengths and a wide choice of emission filters enable imaging of a variety of fluorophores across visible spectrum
- Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample (i.e. Cy2, Cy3, and Cy5)
- Storage phosphor autoradiography delivers high-resolution imaging and accurate quantitation of 3H,14C,125I, 32P, 33P, 35S, and other sources of ionizing radiation
- Typhoon 9410 imager can image microarrays and in situ slides at 10 micron resolution
- The Typhoon exhibits outstanding linearity (4-5 orders of magnitude), quantitative accuracy, and extremely low limits of detection
|Scan area||35 × 43 cm|
|Sensitivity (limit of detection)||Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): <2 dpm/mm2;32P: 5-10-fold lower than 14C
488 nm excited fluorescence: 100 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence: 200 amol HEX, TMR, ROX, and 400 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence: 200 amol Cy5 end-labeled DNA primer in 0.4 mm thick, 12% polyacrylamide gel with TBE buffer.
|Pixel size||1000, 500, 200, 100, 50, 25 (9400 model), and 10 µm (9410 model), selectable|
|Spatial resolution||Autoradiography:2 line pairs/mm for lines drawn with14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mm
|Uniformity||± 5% over entire scan area.|
|Pixel accuracy||± 0.15%|
|Data format||16-bit (65,536 levels) TIFF|
|Linear dynamic range and linearity||Five orders of magnitude (100,000:1) with less than 7.5% relative standard deviation for entire dynamic range.|
|Red light source||Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10,000 h Wavelength: 632.8 nm|
|Green light source||Type: 20 mW solid-state doubled frequency SYAG laser Estimated average lifetime: ~10.000 h Wavelength: 532 nm|
|Blue light source||Type: 30 mW all blue lines Argon ion laser Estimated average lifetime: ~5,000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW)|
|External interface||10 Base-T Ethernet using TCP/IP protocol|
|Software||Scan control software for Windows™ operating system.|
|Power requirements||115/230 V (switchable), 50-60 Hz, < 500 W|
(W × D × H)
|Instrument: 118 × 78 × 48 cm
Blue laser module: 30 × 78 × 48 cm